Critical Factors in Measuring Angiogenesis Using the Aortic Ring Model
نویسندگان
چکیده
منابع مشابه
Critical Factors in Measuring Angiogenesis Using the Aortic Ring Model.
Angiogenesis is a feature of numerous pathologies including cancer and inflammatory conditions and as such is key therapeutic target for the treatment of disorders where excessive or insufficient formation of new blood vessels occurs. The study of angiogenesis in vivo provides many challenges, however the growth of new blood vessels in vitro from aortic explants has provided a highly useful mod...
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Quantification of Angiogenesis on the Rat Aortic Ring Assay
Image analysis is used to quantify angiogenesis on the rat aortic ring model. This technique allows to determine: (1) the aortic ring area and factor shape; (2) the number of microvessels, the total number of branching, the maximal microvessel length and the number of microvessels in function of the distance to the aortic ring; (3) the total number of isolated fibroblast-like cells and the numb...
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The aortic ring model has become one of the most widely used methods to study angiogenesis and its mechanisms. Many factors have contributed to its popularity including reproducibility, cost effectiveness, ease of use and good correlation with in vivo studies. In this system aortic rings embedded in biomatrix gels and cultured under chemically defined conditions generate arborizing vascular out...
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ژورنال
عنوان ژورنال: Journal of Genetic Syndromes & Gene Therapy
سال: 2013
ISSN: 2157-7412
DOI: 10.4172/2157-7412.1000147